Transformation, Transduction and Conjugation (Horizontal Gene Transfer in Bacteria)

Hey Friends,

Transformation, Conjugation and Transduction are ways of bacteria to transfer genetic material horizontally. Transformation is the uptake of free genetic material. Conjugation is exchange of DNA via cell-cell contact and Transduction is gene transfer via a viral vector. In the lab, we use Heat-shock transformation to make bacteria competent to take up plasmid DNA or we use lentiviral transduction (Lentivirus) to integrate genes in the genome of eukaryotic cells.

Cheers
Henrik


References:

Bacterial Transformation:
https://www.thermofisher.com/de/de/home/life-science/cloning/cloning-learning-center/invitrogen-school-of-molecular-biology/molecular-cloning/transformation/bacterial-transformation-workflow.html
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/#B4

Transduction:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC169409/
https://www.nature.com/articles/280080a0

Bacterial Conjugation:
https://www.ncbi.nlm.nih.gov/books/NBK21942/

Cool & Short Blog article:
https://blog.addgene.org/plasmids-101-transformation-transduction-bacterial-conjugation-and-transfection



Chapters:
0:00 Introduction
0:54 Transformation
2:43 Transduction
4:09 Conjugation