Heat-Shock Protocol for Bacterial Transformation 🔬
Learn the heat-shock method to introduce genes into chemically competent bacteria after CaCl₂ treatment.
Henrik's Lab
91.6K views • Apr 19, 2021
About this video
Hey Friends,
the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.
Transformation, Conjugation and Transduction:
https://www.youtube.com/watch?v=7tLV20dk-FM
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681917/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
Thanks
Henrik
Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro
the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.
Transformation, Conjugation and Transduction:
https://www.youtube.com/watch?v=7tLV20dk-FM
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681917/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
Thanks
Henrik
Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro
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Video Information
Views
91.6K
Likes
1.7K
Duration
3:37
Published
Apr 19, 2021
User Reviews
4.7
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