Heat-Shock Protocol for Bacterial Transformation 🔬
Learn the heat-shock method to introduce genes into chemically competent bacteria after CaCl₂ treatment.
Henrik's Lab
91.6K views • Apr 19, 2021
About this video
Hey Friends,
the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.
Transformation, Conjugation and Transduction:
https://www.youtube.com/watch?v=7tLV20dk-FM
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681917/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
Thanks
Henrik
Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro
the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.
Transformation, Conjugation and Transduction:
https://www.youtube.com/watch?v=7tLV20dk-FM
References:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5681917/
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/
Thanks
Henrik
Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro
Tags and Topics
Browse our collection to discover more content in these categories.
Video Information
Views
91.6K
Likes
1.7K
Duration
3:37
Published
Apr 19, 2021
User Reviews
4.7
(18) Related Trending Topics
LIVE TRENDSRelated trending topics. Click any trend to explore more videos.
No specific trending topics match this video yet.
Explore All Trends