Measuring Protein Levels with BCA Assay π§ͺ
Learn how to determine protein concentration using the widely used BCA Protein Assay in your lab experiments.

Henrik's Lab
84.6K views β’ Feb 28, 2022

About this video
A lot of assays are used in the lab to determine the concentration of protein in a sample. One of the most commonly used techniques to quantify the protein concentration is the BCA assay.
References:
BCA molecule: https://en.wikipedia.org/wiki/Bicinchoninic_acid#/media/File:Structure_of_bicinchoninic_acid.png
https://creativecommons.org/licenses/by-sa/3.0/
https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/chemistry-protein-assays.html
https://www.citeqbiologics.com/difference-between-bca-bradford-protein-assays/
Support:
SUBSCRIBE: https://www.youtube.com/channel/UCLwhUgAMJwi9nEoM1Stjt5A?sub_confirmation=1
TWITTER: @HenriksLab https://twitter.com/HenriksLab
LINKED IN: https://www.linkedin.com/in/henrik-ellinghaus-7326021b0/
Music:
Loopop - Subharmonic Bliss (YouTube Audio Library)
https://www.youtube.com/watch?v=gYJwy2C4QQs
Thanks for reviewing: Roman D.
https://twitter.com/RomanMDoll
Time Codes:
0:00 Introduction
0:28 BCA assay vs Bradford method
0:49 BCA protein assay
3:00 Data analysis
#BCA #protein #concentration
References:
BCA molecule: https://en.wikipedia.org/wiki/Bicinchoninic_acid#/media/File:Structure_of_bicinchoninic_acid.png
https://creativecommons.org/licenses/by-sa/3.0/
https://www.thermofisher.com/de/de/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/chemistry-protein-assays.html
https://www.citeqbiologics.com/difference-between-bca-bradford-protein-assays/
Support:
SUBSCRIBE: https://www.youtube.com/channel/UCLwhUgAMJwi9nEoM1Stjt5A?sub_confirmation=1
TWITTER: @HenriksLab https://twitter.com/HenriksLab
LINKED IN: https://www.linkedin.com/in/henrik-ellinghaus-7326021b0/
Music:
Loopop - Subharmonic Bliss (YouTube Audio Library)
https://www.youtube.com/watch?v=gYJwy2C4QQs
Thanks for reviewing: Roman D.
https://twitter.com/RomanMDoll
Time Codes:
0:00 Introduction
0:28 BCA assay vs Bradford method
0:49 BCA protein assay
3:00 Data analysis
#BCA #protein #concentration
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Video Information
Views
84.6K
Likes
1.8K
Duration
4:10
Published
Feb 28, 2022
User Reviews
4.7
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